BECN1 (beclin 1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis.
BECN1, which associates with Bcl-2, plays a significant role in autophagy.
Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated.
Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the ribosomal protein S6, and both are mediated via amino acids.
BECN1 expression in various carcinoma cell lines, such as MCF7, is low, whereas it is ubiquitously expressed in normal breast tissue.
Additionally, BECN1 shares structural similarity to the yeast autophagy gene product, APG6, and was one of the first mammalian proteins discovered to mediate autophagy.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human Beclin 1. Species Crossreactivity: mouse and rat
Clone # USB：R238
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human Beclin 1.
