HDAC and silent information regulator (SIR)-2-dependent acetylation of Bcl-6 causes downregulation of activity by inhibiting the ability of Bcl-6 to recruit complexes containing histone deacetylases (HDACs).
Bcl-6 is frequently deregulated in non-Hodgkin’s B cell lymphomas.
Bcl-6b (B-cell CLL/lymphoma 6, member B), also known as ZNF62, BAZF or ZBTB28, is a 480aa nuclear protein that contains one BTB (POZ) domain and five l C2H2---type zinc fingers.
Expressed ubiquitously with highest expression in placenta and heart, Bcl-6b associates with Bcl-6 and functions as a sequence-specific transcriptional repressor that is thought to be necessary for early B-cell development.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:50-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human BCL6. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human BCL6.
