Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles.
V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP.
A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase.
Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain.
Like F-ATPase, V-ATPase most likely operates through a rotary mechanism.
In yeast, the V-ATPase G subunit is a soluble subunit that shares homology with the F-ATPase G subunit and may be part of a connection stalk between V1 and V0.
The G2 isoform of the G subunit associates with the pore-forming a1C-subunit of L-type calcium channel and aids in proper membrane targeting of the calcium channel.
The genes encoding the G1 and G2 V-ATPase subunits map to chromosomes 9q33.1 and 6p21.3, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human ATP6V1G3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human ATP6V1G3.
