Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles.
V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP.
A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, comprise the V-ATPase complex.
Nine subunits (A–H) make up the V1 domain and five subunits (A, D, C, C' and C") make up the V0 domain.
As part of the V0 domain, V-ATPase A2 (ATPase, H+ transporting, lysosomal V0 subunit a2), consists of 856 amino acids and is also known as ATP6V0A2, V-type proton ATPase subunit a isoform 2, vacuolar proton translocating ATPase subunit a isoform 2, lysosomal H(+)-transporting ATPase V0 subunit a2 or TJ6.
V-ATPase A2 is a multi-pass membrane protein with localization in the cell membrane, endosome membrane and the subapical vesicles of the kidney’s proximal tubules.
V-ATPase A2 plays an important role in Golgi function by regulating pH.
Wrinkly skin syndrome (WSS) and cutis laxa type II (ARCL type II) are caused as a result of V-ATPase A2 defects.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human ATP6V0A2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to residues in human ATP6V0A2.
