Atg14 (ATG14 autophagy related 14 homolog), also known as BARKOR (beclin 1-associated autophagy-related key regulator) or ATG14L, is a 492aa protein that contains 3 coiled-coil domains in its N-terminal region.
Conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly and mosquito, Atg14 exhibits highest expression in brain and lowest expression in kidney and ovary.
Atg14 localizes to isolation membranes of forming autophagosomes, cytoplasm, endoplasmic reticulum and an uncharacterized punctate structure.
Necessary for both basal and inducible autophagy, Atg14 plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine.
Atg14 promotes BECN1 translocation from the trans-Golgi network to autophagosomes, and forms a complex with BECN1, PI 3-kinase p100 and p150.
Atg14 does not form complexes with KIAA0226/Rubicon or UVRAG, which forms a mutually exclusive complex with BECN1 through direct competition with Atg14.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ATG14. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human ATG14.
