Serotonin, a monoamine neurotransmitter associated with neuronal modulation of emotions, is synthesized by serotonergic neurons of the central nervous system through metabolism of the essential aa L-tryptophan.
In the pineal gland, serotonin can be transformed into the circadian regulatory hormone melatonin by ASMT (acetylserotonin O-methyltransferase) which catalyzes the final reaction in the synthesis pathway.
ASMTL (N-acetylserotonin O-methyltransferase-like protein) is a 621aa protein with a putative catalytic S-adenosyl-L-methionine domain that shares high genetic homology to ASMT.
The ASMTL protein is encoded by a gene from the PAR1 region of the X and Y chromosomes, which is considered to be a fusion product of two evolutionarily disparate genes.
ASMTL, with potential cytoplasmic localization, is abundant in pancreas, placenta, fibroblast, thymus, prostate, testis, ovary and colon.
Low expression levels are found in spleen, small intestine and leukocytes.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ASMT.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ASMT.
