ASF1A (anti-silencing function 1A) is a transcription factor that has been shown to bind with HeLa cell core Histones H3 and H4.
Human ASF1A, which shares 71%aa identity to ASF1B, is believed to act as a chaperone to derepress genes since that action is observed in yeast Asf1A.
High phosphorylation of ASF1A is demonstrated during the S-phase of the cell cycle.
Both ASF1A and B are phosophorylated by the proteins TLK1 and TLK2.
The ASF1B gene has been mapped to chromosome 19p13.13 while the ASF1A gene localizes to 6q22.31.
ASF1A and B cooperate in the formation of nucleosomes along with the protein CAF1.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ASF1A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human ASF1A.
