84-4378-35　［取扱停止］ASAP2 (Arf-GAP with SH3 Domain, ANK Repeat and PH Domain-containing Protein 2, Development and differentiation-enhancing Factor 2, Paxillin-Associated Protein with ARF GAP Activity 3, PAG3, Pyk2 C-terminal-Associated Protein, PAP, DDEF2, KIAA0400) 50ul　359819

特徴

• DDEF2 (ADP ribosylation factor [ARF]- GTPase-activating protein [GAP] containing SH3, ANK repeats, and PH domain, PAP, PAG3, AMAP1, ZG14P, centaurin beta4) is a phospholipid- dependent ADP-ribosylation factor (ARF) GTPase-activating protein (ARF-GAP) that binds to protein-tyrosine kinases Src and focal adhesion kinase.
• ARF family GTP-binding proteins are regulators of membrane traffic and cytoskeletal organization.
• Modulation of ARF activity by DDEF2 is important for the regulation of focal adhesion assembly and/or organization by influencing the mechanisms responsible for the recruitment and organization of focal adhesion proteins paxillin and FAK.
• In spreading platelets, most endogenous DDEF2 is localized at peripheral focal adhesions.
• Pyk2 directly phosphorylates DDEF2 on tyrosine-308 and -782, and this event affects the phosphoinositide binding profile of DDEF2.
• DDEF2 is phosphorylated on tyrosine residues in cells expressing activated Src and tyrosine phosphorylation depends on a proline-rich class II Src SH3 binding site.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.