84-4377-79　［取扱停止］ANTXR1 (Anthrax Toxin Receptor 1, Tumor Endothelial Marker 8, ATR, TEM8) 100ul　359791

特徴

• The tripartite toxin secreted by Bacillus anthraci is the causative agent of anthrax evading the immune system and killing the host during a systemic infection.
• Two components of the toxin, odemema factor (OF) and lethal factor (LF) enzymatically modify substrates within the cytosol of mammalian cells.
• The third component, protective antigen (PA), binds to a cellular receptor, designated ATR (anthrax toxin receptor), which mediates the delivery of the enzymatic components to the cytosol.
• TEM8 (tumor endothelial marker 8) is one of the tumor specific endothelial markers (TEMs) whose N-terminus encodes ATR.
• TEM8 is highly expressed in tumor endothelial cells but not in normal endothelial cells.
• TEMs have elevated expression during tumor angiogenesis.
• Four TEM genes, TEM1, TEM5, TEM7 and TEM8, encode the TEM proteins, which contain putative transmembrane domains.
• ATR is a type I membrane protein with an extracellular von Willebrand factor A domain that binds directly to PA.
• The first 364aa of ATR protein are identical to those of TEM8.
• However, the C-terminal ends of the ATR and TEM8 proteins are different, presumably due to alternative splicing.
• A soluble version of von Willebrand factor A domain seems to protect cells from the toxin action.
• Applications:Suitable for use in Immunofluorescece, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.