Anillin, also known as scraps homolog, is an evolutionarily conserved Actin-binding protein required for cytokinesis that was first identified in Drosophila melanogaster.
Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system.
It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase.
During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin.
In Anillin knockout cells the cleavage furrow fails to complete ingression.
Anillin expression levels fluctuate with the cell cycle, peaking in mitosis.
Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C).
APC/C recognizes the D-box domain at the N-terminal region of Anillin.
Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.
Applications:Suitable for use in Immunofluorescece, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ANLN.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human ANLN.
