Human liver contains isoforms of dihydrodiol dehydrogenase (DD1, DD2, DD3 and DD4), which belong to the aldo-oxo reductase/aldo-keto reductase (AKR) superfamily, have 20a- or 3a-hydroxysteroid dehydrogenase (HSD) activity.
DD1 is also designated AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2.
AKR1C3 and 3a-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 also can be called AKR1C4, CD, CHDR or AKR1C6 (in rodents).
DD1 and DD2 are 20a-HSDs, whereas DD3 and DD4 are the 3a-HSDs.
The multiple human cytosolic dihydrodiol dehydrogenases are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons, pesticides and steroid hormones, and are responsible for the reduction of ketone-containing drugs by using NADH or NADPH as a cofactor.
The 20a-HSD catalyzes the reaction of progesterone to the inactive form 20a-hydroxyprogesterone.
The 3a-HSD is a cytosolic, monomeric, NADPH-dependent oxidoreductase that reduces 3-keto-5-dihydrosteroids to their tetrahydro products.
DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver.
DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzenedihydrodiol and trans-naphthalenedihydrodiol.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:00-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human AKR1C1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human AKR1C1.
