84-4377-25　［取扱停止］AKAP3 (A-Kinase Anchor Protein 3, AKAP-3, A-Kinase Anchor Protein 110kD, AKAP 110, Cancer/testis Antigen 82, CT82, Fibrous Sheath Protein of 95kD, FSP95, Fibrousheathin I, Fibrousheathin-1, Protein Kinase A-Anchoring Protein 3, PRKA3) 100ul　359762

特徴

• The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates.
• Specificity of PKA signaling is mediated by the compartmentalization of the kinase to specific sites within the cell.
• To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAP).
• AKAP 3, also known as AKAP 110, FSP95, PRKA3 and SOB1, binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions.
• Expression of AKAP 3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP 3, thereby increasing its recruitment of PKA.
• AKAP-3 also exhibits high expression in patients with epithelial ovarian cancer (EOC).
• It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, which make AKAP 3 a potential target for antigen-specific immunotherapy in EOC.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.