Activation-induced Cytidine Deaminase (AID, HIGM-2) is a 198aa, RNA-editing enzyme that contains a conserved cytidine deaminase motif and plays an important role in B-cell terminal differentiation.
AID is expressed in germinal center B cells and contributes to the production of neutralizing antibodies IgG, IgA, and IgE.
Hyper-IgM syndrome (HIGM2) patients that have deficient levels of AID show the absence of immunoglobulin class switch recombination (CSR), lack of immunoglobulin somatic hypermutations, and lymph node hyperplasia mediated by the presence of giant germinal centers.
Furthermore, AID-/- mice are defective in CSR and also show a hyper-IgM phenotype, characterized by enlarged germinal centers containing active B cells.
AID thus appears to be required in several stages of B-cell terminal differentiation that are necessary for efficient antibody responses such as B cell proliferation, immunoglobulin somatic hypermutations and CSR.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human AICDA. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to residues in human AICDA.
