84-4377-08　［取扱停止］AFF1 (AF4/FMR2 Family Member 1, ALL1-Fused Gene from Chromosome 4 Protein, Protein AF-4, Protein FEL, Proto-oncogene AF4, AF4, FEL, MLLT2, PBM1) 50ul　359754

特徴

• Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, which is associated with acute leukemias.
• The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor in which AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors.
• MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia.
• The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.