ADAMTS (a disintegrin and metalloproteinase domain with Thrombospondin type-1 modules) is a family of zinc-dependent proteases that are implicated in a variety of normal and pathological conditions, including arthritis and cancer.
ADAMTS protein family members contain an amino-terminal propeptide domain, a metalloproteinase domain, a disintegrin-like domain and a carboxy-terminus that contains a varying number of thrombospondin 1 (TSP 1) motifs.
ADAMTS genes are primarily expressed in fetal tissues, including the lung, kidney and liver.
ADAMTS-18 is a secreted protein that is also expressed in adult brain, endothelium, prostate and submaxillary gland.
ADAMTS-18 has two known splice variants and it is structurally most similar to ADAMTS-16.
ADAMTS-18 is downregulated in carcinoma cell lines by methylation of its promoter.
Forced expression of ADAMTS-18 in carcinoma cells can lead to significant inhibition of cell growth, suggesting that it plays a significant role as a tumor suppressor.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human ADAMTS18. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human ADAMTS18.
