The ADAM (a disintegrin and metalloprotease) protein family, which includes over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases, plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFa (tumor necrosis factor a), and modulation of the neurogenic function of Notch and Delta.
The ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail.
ADAMs are expressed in a wide range of mammalian tissues and several are abundantly expressed in the male reproductive tract.
Expression of ADAM19, also designated meltrin b, is highest in the peripheral nervous system during embryogenesis, but is also apparent in placenta, brain, heart, lung, leukocytes, and SW480 cells.
ADAM19 also serves as a dendritic cell marker.
Truncation of ADAM19 in its cysteine-rich domain is necessary to exert its proteolytic activity on specific substrates, including a2-macroglobulin.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human ADAM19.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues in human ADAM19.
