The SbmA protein is a homodimeric secondary transporter protein, that is involved in the transport of microcins B17 and J25, glycopeptide antibiotic bleomycin, proline-rich antimicrobial peptides of prokaryotic and eukaryotic origin, antisense peptide phosphodiamidate morpholino oligomers and peptide nucleic acids (PNA) into the E.
coli cytoplasm.
This protein is found in a large number of Gram-negative and also Gram-positive microorganisms, including plant and animal pathogens.
The monomeric SbmA protein contains 406aa (about 50kD) and has 8 transmembrane regions.
The homodimeric complex present in the membrane closely resembles the membrane-spanning region of the ATP-binding cassette transporter family.
Peptide transport mediated by SmbA is driven by a electrochemical gradient.
SbmA-mediated internalization of peptide substrates suggest that the transport of an unknown substrate(s) represents the function of this protein.
Applications:Suitable for use in Western Blot amd Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: The band size to be expected is ~37kD under reducing conditions
Optimal dilutions to be determined by the researcher.
Positive Control:Recombinant SbmA or E.
coli lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide.
Specificity：Recognizes the inner membrane protein SbmA of Gram-negative bacteria.
Isotype：IgG
Calc Applications Abbrev：IP WB
Immunogen：Recombinant protein corresponding to SbmA.
