ICAM-1 permits antigen- independent adhesion between lymphocytes and their targets.
The LFA-1 binding site is located in domain 1 of ICAM-1.
The CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system.
CD54 is weakly expressed on resting peripheral blood lymphocytes.
Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.
CD54 is the receptor for rhinoviruses and malaria.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (frozen): staining of ICAM-1 in frozen tissue sections.
Useful for staining chimpanzee, rhesus monkey, cynomolgous monkey and baboon tissues
Optimal dilutions to be determined by the researcher.
Use:For selection of the most useful dilution in a given situation, a test staining with cells or tissue known to express the antigen should be performed.
To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37°C.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide.
Specificity：Recognizes both intact human ICAM-1, CD54 and with soluble human ICAM-1. Species Crossreactivity: baboon, chimpanzee, cynomolgus monkey, rhesus monkey.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mk
Immunogen：Recombinant protein corresponding to human ICAM-1.
