Antimicrobial proteins (AMP) are a fundamental element of the primary response against pathogens.
AMP’s are small endogenous cationic molecules expressed by phagocytic and epithelial cells.
The antimicrobial activity of AMP’s is directed towards a broad spectrum of pathogens, like Gram-positive & negative bacteria, viruses, yeast and fungi.
AMP’s aid in innate and adaptive immunity via direct inactivation and by immunomodulatory activity like leukocyte migration.
Defensins are the most prominent mammalian AMP’s.
Three defensin peptide families are identified, the alpha-, beta-, and theta-defensins.
They are characterized by a triple-stranded beta-hairpin structure, six disulfide-linked cysteine residues and a positive charge.
They are synthesized as preproteins and undergo processing to become a fully active peptide.
Defensins are divided in alpha- and beta-defensins depending on their disulfide bridging pattern.
alpha- and beta-defensins modify cell migration and maturation, induce cytokines and trigger histamin and prostaglandin D2 release from mast cells.
There are six alpha-defensins: HNP (human neutrophil peptide)-1 to -4, HD5 & 6.
Human beta-defensin 1(hBD-1) is considered a prominent AMP in epithelial defense against infection.
High expression levels have been found in the kidney and female reproductive tract, especially in pregnant women.
Besides antimicrobial activity, hBD1 has an effect on chemoattractive immature dendritic cells and probably memory T cells.
After reduction of disulphide-bridges hBD-1 becomes a potent antimicrobial peptide against the opportunistic pathogenic fungus Candida albicans and against anaerobic Gram positive commensals of Bifidobacterium and Lactobacillus species.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: typical starting working dilution is 1:50, size to be expected under reducing conditions is ~3.9kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide.
Specificity：Recognizes human beta-defensin 1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human beta-defensin 1.
