XIAP, also known as IAP3 or BIRC4, is a protein that stops apoptotic cell death.
It is mapped to chromosome Xq25.
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins.
Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function.
This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme.
This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.
Mutations in this gene are the cause of X-linked lymphoproliferative syndrome.
Alternate splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to ~0.5mg/ml.
Specificity：Recognizes human XIAP. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to A15-V244 of human XIAP expressed in E. coli. Species sequence homology: mouse (89.4%) and rat (90.7%)
