Thymidine kinase 1, soluble (gene name TK1), is a human thymidine kinase.
Thymidine kinase catalyzes the phosphorylation of thymidine to deoxythymidine monophosphate.
This gene is mapped to 17q23.2-q25.3.
It is estimated that the maximal size of the TK1 gene is 14kb and its minimal size between 4 and 5 kb.
The gene contains many noncoding inserts and numerous Alu sequences.
There are two forms of this protein, which have been identified in animal cells, one in cytosol and one in mitochondria.
Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to ~0.5mg/ml.
Specificity：Recognizes human TK1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to S2-N234 of human TK1 expressed in E. coli. Species sequence homology: mouse (87.1%) and rat (91.7%).
