T-complex protein 1 subunit alpha is a protein that in humans is encoded by the TCP1 gene.
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC).
This complex consists of two identical stacked rings, each containing eight different proteins.
Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner.
The complex folds various proteins, including actin and tubulin.
Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.
In addition, three pseudogenes that appear to be derived from this gene have been found.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to ~0.5mg/ml.
Specificity：Recognizes human TCP1 alpha. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa515-551, KFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHS, of humsn TCP1 alpha at C-terminal, different from the related mouse sequence by 1aa and from the related rat sequence by 2aa.
