Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene.
It is localized on 8p11.2.
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis.
It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB.
The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage.
Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Additionally, human POLB forms a complex with and is methylated by PRMT6.
In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme.
The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to ~0.5mg/ml.
Specificity：Recognizes human POLB.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to L122-E335 of human POLB expressed in E. coli. Species sequence homology: mouse (95.3%) and rat (94.9%)
