Matrix Gla protein (MGP) is an 84-residue vitamin K-dependent protein initially isolated from bovine bone.
In addition, MGP is a 10kD protein produced and secreted by vascular smooth muscle cells and chondrocytes and significantly accumulated in bone, cartilage, and dentin.
It is also expressed at high levels in heart, kidney, and lung and is upregulated by vitamin D in bone cells.
MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins.
The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization.
And this gene is mapped to 12p12.3.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to ~0.5mg/ml.
Specificity：Recognizes human MPG.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Y20-F96 of human MGP expressed in E. coli. Species sequence homology: mouse (84.4%) and rat (80.5%)
