Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene.
This gene is a member of high affinity glutamate transporter family.
It is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH).
And this gene transports L-glutamate and also L- and D-aspartate.
It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft.
Furthermore, this gene acts as a symport by cotransporting sodium.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Boiling paraffin sections in 10mM citrate buffer, pH 6.0, for 20min is required for staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 5% BSA, 0.9% sodium chloride, 0.2% Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human EAAT1. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa14-42, RMERFQQGVRKRTLLAKKKVQNITKEDVK, of human EAAT1 at N-terminal, different from the related mouse sequence by 3aa and identical to the related rat sequence.
