84-4375-46　CDCP1 (CUB Domain-Containing Protein 1, CD 318, CD318, CD318 Antigen, CDCP 1, SIMA 135, SIMA135, TRASK) 100ug　353244

特徴

• CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene.
• It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases).
• CDCP1/Trask is a 140kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines.
• The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion.
• The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment.
• The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype.
• CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (Paraffin): 0.5-1ug/ml.
• Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutess is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.