CD9 antigen is a protein that in humans is encoded by the CD9 gene.
CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins.
It is found on the surface of exosomes.
It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.
In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization.
While oocytes are ovulated, CD9- deficient oocytes are not properly fused with sperm upon fertilization.
CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to ~0.5mg/ml.
Specificity:Recognizes mouse CD9.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Mo
Immunogen:Recombinant protein corresponding to T110-I193 of mouse CD9 expressed in E. coli. Species sequence homology: human (77.4%) and rat (86.9%)