84-4375-24　AIFM1 (Apoptosis-Inducing Factor 1, Mitochondrial, PDCD8, AIF, Apoptosis Inducing Factor, Apoptosis-Inducing Factor 1, CMTX4, COXPD6) 100ug　353222

特徴

• Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene.
• AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence.
• This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells.
• Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation.
• In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
• Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.
• A related pseudogene has been identified on chromosome 10.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/mlImmunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.