84-4375-19　ZP2 (Zona Pellucida Sperm-Binding Protein 2, Zona Pellucida Glycoprotein 2 (Sperm Receptor), Zp-2) 100ug　353132

特徴

• Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene.
• The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida.
• It is also responsible for the primary block to polyspermy in mammals.
• The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin.
• After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space.
• Ovastacin cleaves ZP2 at the N-terminal, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida.
• Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases.
• Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.