84-4374-77　Filaggrin (Epidermal Filaggrin, FILA, Filaggrin Precursor, Profilaggrin, bFGF-R-1, FGFBR, FLG, FLT2, FGFR1, FLG, FLT-2, Fms-like Tyrosine Kinase 2, Proto-oncogene c-Fgr) 100ug　352862

特徴

• In humans, profillagrin is encoded by the FLG gene, which is part of the S100 fused-type protein (SFTP) family within the epidermal differentiation complex on chromosome 1q21.
• The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis.
• It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.
• Mutations in this gene are associated with ichthyosis vulgaris.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (Paraffin): 0.5-1ug/ml.
• Heat induced antigen retrieval with 10mM sodium citrate buffer, pH 6.0 for 20 minutes is required for staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.