Cathepsin D is a protein that in humans is encoded by the CTSD gene.
This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A.
It is mapped to 11p15.5.
The cDNA encodes a 412aa protein with 20 and 44aa in a pre and prosegment, respectively.
Cathepsin D is one of the lysosomal proteinases.
It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis.
Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human Cathepsin D.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Cathepsin D expressed in E. coli. Species sequence homology: mouse (84.4%) and rat (84%).
