84-4374-50　Apolipoprotein A I (Apo AI, APOA 1, ApoA I, APOA1, ApoA-I, Apo-AI, Apolipoprotein A I, Apolipoprotein A1, Apolipoprotein A,| Apolipoprotein A-I, Apolipoprotein A-I (1-242), ProapoA-I, Proapolipoprotein A-I, Truncated apolipoprotein A-I) 100ug　352713

特徴

• Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism.
• It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL.
• The gene is mapped to 11q23.
• And it is a single polypeptide chain with 243aa of known primary amino acid sequence.
• The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion.
• It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters.
• ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect.
• Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.
• Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (FFPE): 0.5-1ug/ml.
• Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.