The ion channels activated by glutamate are typically divided into two classes.
Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR).
The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain.
The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD
Western blot analysis of GluR1 transfected cells and rat brain homogenates the antibody specifically labels a single band at ~102kD.
Western blot analysis of GluR2, 3, 4, 4C, 5, 6, and 7 transfected cells revealed no immunolabeling.
Immunolabeling of the above non-NMDA transfected cells demonstrates specificity for GluR1.
Additionally, immunolabeling for GluR1 is completely abolished by pre-adsorption with synthetic rat GluR1 aa894-907 at 5ug/ml of diluted antibody.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder.
Specificity：Recognizes rat GluR1.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to aa894-907 of rat GluR1 coupled to bovine thyroglobulin (BTg) with glutaraldehyde at C-terminal.
