The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains.
It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1(EBF1) in erythroid cells and Runx2 in osteoblast precursors.
ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Kidney Tissue Slide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human ZNF521.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 19aa from human ZNF521 at C-terminal.
