YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue.
This localization plus the fact that the family of human YPEL proteins share a high degree ofsequence homology across species suggests that these proteins may have a conserved function involved in cell division.
YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis.
Finally, during cytokinesis, YPEL5 is localized to the midbody.
It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 2.5ug/mlWestern Blot: 1ug/mlImmunocytochemistry: 2.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A-20 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human YPEL5. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 14aa from human YPEL5 at N-terminal.
