The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1).
It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2).
WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3).
Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Colon Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human WSB1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to 16aa from human WSB1 near N-terminal.
