The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1).
UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2).
UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding.
Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3).
UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4,5).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Lung Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human UVRAG. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 18aa near the C-terminal of human UVRAG.
