USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76aa protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1).
It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats.
Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2).
The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function.
USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human USP25. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 17aa from human USP25.
