TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development.
It is a 526aa nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes.
TOX-mediated positive selection is associated with upregulation of RUNX3 and is calcineurin dependent.
TOX-dependent transition to the CD4+CD8 stage is required for development of class II majorhistocompatibility complex-specific T cells.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/ml Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Colon Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human TOX.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa from human TOX at N-terminal.
