TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1).
TESPA1 encodes a protein of 458aa and is highly conserved among vertebrate species (2).
TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3).
TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mL.
Western Blot: 1-2ug/mlImmunocytochemistry: 5ug/mL
Optimal dilutions to be determined by the researcher.
Positive Control:A431 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human TESPA1.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to 18aa near the N-terminal of human TESPA1.
