The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH).
Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene.
In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished.
Furthermore, high levels of angiogenic factors EPAS1 and VEGF wasobserved, which may stimulate tumor growth.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 2.5ug/mlWestern Blot: 1-2ug/mlImmunocytochemistry: 2.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control:EL4 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human SDHD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa from human SDHD.
