Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein.
Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction.
Pst1 is a protein with 444aa and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor.
Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated.
It migrates at ~200kD on SDS-PAGE when produced in wild type S.
cerevisiae.
Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S.
cerevisiae stain (deltaoch1 deltamnn1 deltamnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at ~100kD.
When produced in the yeast, Pst1 can be recognized by several glycan specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies.
Among several heavily N-glycosylated yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1ug/mlWestern Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes yeast Pst1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ye
Immunogen：Synthetic peptide corresponding to yeast Pst1 at N-terminal.
