PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins.
It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus.
Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation.
Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 5ug/mlWestern Blot: 0.25ug/mlImmunocytochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A549 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PRICKLE4.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 18aa from human PRICKLE4 at N-terminal.
