PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired- homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis.
PHOX2 proteins are crucial for the regulation of endogenoushydroxylases in neural crest cells and promote sympathetic neuron generation.
Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain.
In contrast, PHOX2A controls only thedifferentiation of the main noradrenergic center of the brain.
Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:293 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PHOX2B. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa from human PHOX2B.
