PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes.
They possess five immunoglobulin like domains that are involved in ligand binding.
Two tyrosine kinase domains are separated by a kinase insert to which PI-3 kinase can bind.
PDGF AA can bind autophosphorylate and activate only PDGFR, alpha while PDGF BB can bind and activate to both PDGFR, alpha and beta.
Platelet-derived Growth Factor Receptor Alpha (PDGFR alpha), a member of the a PDGF Receptor tyrosine kinase family, is a mediator of the biological actions of platelet-derived growth factor (PDGF).
PDGF binding to PDGF receptors result in stimulation of cell growth, chemotaxis, and cell shape changes.
Unlike PDGFR beta, PDGFR alpha can bind to both PDGF subunits.
PDGFR alpha has been implicated in the development of spina bifida.
In various mouse models, deregulated PDGFR alpha expression was found to result in congenital neural tube defects.
In humans, various PDGFRA promoter haplotypes have been associated with neural tube defects.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin/paraffin): use at 5-10ug/ml for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Ovarian carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：125ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human PDGFRA. Species Crossreactivity: mouse and rat. Does not react with PDGFR, beta.
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PDGFRA at C-terminal. Cellular Localization: Cell membrane
