Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2).
PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3).
Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4).
Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PDCL3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 14aa from human PDCL3 at C-terminal.
