OASL (2'-5'-oligoadenylate synthetase-like), also known as p59OASL or TRIP14 (thyroid receptor-interacting protein 14), is a 514aa protein that exists as two alternatively spliced isoforms, designated p56 and p30, and contains two ubiquitin-like domains (1).
It is widely expressed in a variety of tissues and interacts with the ligand binding domain of the thyroid receptor (TR) and is able to bind double-stranded RNA and DNA, possibly playing a role in RNA degradation and the overall inhibition of protein synthesis (2-3).
Methyl CpG-binding protein 1 (MBD1), which functions as a transcriptional repressor, was identified as a strong p59 OASL interactor (4).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunocytochemistry: 2.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Bladder Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human OASL. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 17aa from human OASL at N-terminal.
