Mucin 1 (MUC1) is membrane-bound protein member of the mucin family.
Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces and also play a role in intracellular signaling (1).
MUC1 is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas.
MUC1 is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex.
The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling.
Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas (1).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry (paraffin).
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human MUC1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 17aa near the C-terminal of human MUC1.
