The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1).
MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1).
It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunocytochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A431 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human MIB1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 19aa near the C-terminal of human MIB1.
