KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+channels subfamily.
The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity.
Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells.
KCNK12 is expressed mainly in the brain and lung, but also observed in the kidneys, heart and skeletal muscle.
KCNK12 is closely related to KCNK13 (58% identity at the aa level), but could not yet been functionally expressed in vitro and may require other proteins to become active.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 0.5ug/mlImmunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human KCNK12. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 14aa from human KCNK12 at C-terminal.
